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KMID : 0383419680090020267
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Korean Modern Medical Journal
1968 Volume.9 No. 2 p.267 ~ p.275
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Effect of Fracture and Administration of Corticosteroid on the Incorporation of ^(32)P in Bone
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Abstract
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A series of experiment was conducted with male mice to observe the effect of corticosteroid n metabolism of fractured bone.
One hundred and eighty mice were divided into two groups; one in vivo group and the other n vitro group.
Each of these were further subdivided into control, cortisone and DOCA groups.
(A) ln vivo experiment;
Corticosteroid (cortisone acetate 0.5mg/day, DOCA 0. ling/day) were injected subcutaneously or three successive days and closed, complete fracture of left femur was induced manually imme-$iately following final administration of corticoid. Ten mice each were sacrificed at 2, 4 and 6 days fracture. All femurs, fracture and intact, were immersed for 24 hours in solution of the {rebs-Ringer phosphate containing two microcurie of radioactive phosphorus.
(B)In vitro experiment:
A complete simple fracture of left femur was incurred in each of 90 mice and these animals were sacrificed, at the various time intervals following fracture as in vivo´ experiment.
All femurs were incubated with the Krebs-Ringer phosphate solution (containing ZaC H332PO4) room temperature for 48 hours. The femurs of corticosteroid groups were treated with 1.5mg ´cortisone acetate and 0.3mg of DOCA.
In all experiments, the colorimetric determination of phosphorus was made after the method ribed Fiske, Subbarrow and radioactivity measurement was obtained by Geiger counter. The wing results were obtained;
(1) No significant changes in phosphorus level of bone were noted between fracture and acture, or between in vivo and in vitro.
rvals after fracture likewise had no appreciable effect on the level of phosphorus.
(2) Specific activities of the phosphorus were increased in fractured bones DOCA groups decreased in cortisone groups.
It can be assumed that incorporation of bone salt is accelerated by fracture and administr) of DOCA while it is inhibited by cortisone.
(3) The similar pattern was also found in specific activity of phosphorus in both vivo vitro, experiments. From the above experiment, it can be concluded that the effect of corticost on bone metabolism is direct.
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KEYWORD
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